Expression and significance of P53 protein and MDM-2 protein in human gliomas / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>P53 is one of the most studied tumor suppressors in the cancer research, and over 50% of human tumors carry P53 mutations. MDM-2 is amplified and/or overexpressed in a variety of human tumors of diverse tissue origin. The aim of this study was to examine the expression of P53 protein and MDM-2 protein in gliomas, and to investigate the relationship between the expression of the two proteins and the histopathological grades of glioma. The relationship between MDM-2 protein expression and P53 protein expression was also analyzed.</p><p><b>METHODS</b>The expression of P53 protein and MDM-2 protein was immunohistochemically detected using monoclonal antibodies in 242 paraffin embedded tissues, including 30 normal brain tissues from patients with craniocerebral injury and 212 tissues from patients with primary glioma (grade I - II group: 5 cases of grade I, 119 cases of grade II; and grade III--IV group: 53 cases of grade III, and 35 cases of grade IV).</p><p><b>RESULTS</b>The P53 positive rate was significantly higher in the glioma groups than in the control group (P < 0.0001). The P53 positive rate was significantly higher in glioma tissues of grade III - IV than in glioma tissues of grade I - II group (P = 0.001). The MDM-2 positive rate was significantly higher in glioma groups than in the control group (P < 0.0001). There was no significant difference in the MDM-2 positive rate between the two glioma groups (P = 0.936). The expression of P53 protein was not related to expression of MDM-2 protein (P = 0.069)</p><p><b>CONCLUSIONS</b>Overexpression of P53 protein might be related to the occurrence and progression of glioma. Overexpression of MDM-2 protein may play an important role in glioma tumorigenesis, but may not be involved in glioma progression. The overexpression of MDM-2 protein was an early event in malignant transformation of glioma. MDM-2 may be a key player in glioma in its own right.</p>

Clinicopathologic evaluation of 36 cases of allergic fungal sinusitis / 中华病理学杂志

<p><b>OBJECTIVE</b>To characterize clinicopathological features of allergic fungal sinusitis (AFS).</p><p><b>METHODS</b>Thirty-six cases of AFS were retrieved from the department archival files of Beijing Tongren Hospital from 2002 to 2006. AB-PAS, GMS and MUC5B stain were performed using paraffin-embedded tissues of the cases. Ten cases with available fresh diagnostic tissue were investigated by electron microscopy.</p><p><b>RESULTS</b>Patients included 21 males and 15 females. The age of patients ranged from 11 to 53 years. Atopy was very common in these patients. On plain CT scans, the affected nasal sinuses were filled with soft tissue shadow with patchy hyperdensity. The bony sinus wall showed areas of pressure erosion. Skin antigen tests showed fungal positivity in 31 of 36 cases. Serum levels of the total IgE and/or the specific fungal IgE were elevated in 20 cases. The eosinophil quantity was elevated in 23 cases. Fungal culture was positive in 10 cases. Gross examination showed thick putty secretions within the lesions. Light microscopy showed typical "eosinophilic mucin". Fungal elements were seen with AB-PAS, GMS and MUC5B stains. Electron microscopy demonstrated degranulation by the eosinophils.</p><p><b>CONCLUSIONS</b>"Eosinophilic mucin" is the typical histopathological feature of AFS. AB-PAS, GMS and MUC5B staining methods can used to detect fungal species in mucin. Accurate diagnosis of AFS requires correlations among clinical findings, radiologic examinations, laboratory tests and histopathologic features. However, the ultimate diagnosis requires a histopathologic confirmation.</p>

Increased expression of fascin associated with malignant transformation of sinonasal inverted papilloma / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Fascin, an actin binding protein, usually expressed at a low level in normal epithelium, but is significantly increased in transformed epithelial cells and several common carcinomas. In this study, we examined the expression of fascin by immunohistochemistry in sinonasal epithelium with chronic inflammation (control group), exophytic papilloma (EP), inverted papilloma (IP) with dysplasia and cancerated IP (including carcinoma in situ and invasive squamous cell carcinoma, SCC), and furthermore investigated the relationship between fascin expression and formation of malignant IP.</p><p><b>METHODS</b>Fascin expression was immunohistochemically detected using monoclonal antibody against fascin in 86 paraffin embedded tissues, including 10 cases of sinonasal mucosa with chronic inflammation, 10 of EP, 45 of IP with dysplasia (45 cases were divided into three groups: IP with mild dysplasia, IP with moderate dysplasia, and IP with severe dysplasia, 15 cases each), and 21 of cancerated IP.</p><p><b>RESULTS</b>The level of fascin expression was significantly higher in the neoplastic tissue than that in control group. Fascin expression increased gradually with the progression from sinonasal epithelium with chronic inflammation, IP with mild dysplasia, IP with moderate dysplasia, IP with severe dysplasia, to cancerated IP, and significant difference of fascin expression was observed between any two groups of the five.</p><p><b>CONCLUSION</b>Precancerous lesions of IP exhibit elevated levels of fascin that may be associated with carcinogenesis of IP. Fascin may play a role in the formation of IP and EP.</p>

The change of circulating endothelial cell in blood of rats and pulmonary small artery vascular endothelial cell under acute hypoxia / 中国应用生理学杂志

<p><b>AIM</b>To investigate changes of VEC and CEC under acute hypoxia.</p><p><b>METHODS</b>Observe CEC in blood under acute hypoxia morphologically and count the number of CEC by optical microscope, measure LDH activity of young CEC and VEC by histochemical staining image analysis.</p><p><b>RESULTS</b>LDH activities of VEC in hypoxic groups are lower than that in the group before hypoxia and decrease progressively with hypoxia time. LDH activities of young CEC in groups after hypoxia and before hypoxia are the same and are apparently lower than that of VEC. Before hypoxia most of CEC are aging, the number of CEC from hypoxic groups is greater than that before hypoxia and increases progressively with hypoxia time and most of CEC from hypoxic groups are young.</p><p><b>CONCLUSION</b>The morphology and number of CEC may reflect the extent to which the vascular is injured. LDH activity of VEC may reflect the transformation from VEC into CEC. LDH activity of young CEC may reflect the extent to which the VEC is injured when falling from vascular wall.</p>